Description

L-Norleucine-4-Nitroanilide Hydrobromide is a high-purity, synthetic peptide derivative used as a critical biochemical reagent. This compound serves as a specialized chromogenic substrate for the sensitive detection and kinetic study of proteolytic enzymes, particularly aminopeptidases. It is essential for researchers and manufacturers in pharmaceutical development, diagnostic assay production, and advanced biochemical research requiring precise enzyme activity analysis.

Application

• Enzyme Substrate for Aminopeptidases: Primary chromogenic substrate for the colorimetric and spectrophotometric assay of leucine aminopeptidase (LAP) and related enzymes.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize enzyme inhibitors for drug discovery programs.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring aminopeptidase activity in biological samples.
• Biochemical Research: Employed in fundamental enzymology studies to investigate enzyme kinetics, specificity, and mechanism of action.
• Quality Control in Bioprocessing: Monitors proteolytic activity during the production and purification of therapeutic proteins and peptides.
• Food Science & Microbiology: Applied in research to study proteolytic enzyme systems in microorganisms and food processing.

Basic Information

Product Name	L-Norleucine-4-Nitroanilide Hydrobromide
CAS No.	213271-08-0
Molecular Formula	C12H18N4O3 • HBr
Molecular Weight	371.21 g/mol
Synonyms	L-Norleu-4-NA • HBr; L-Norleucine 4-nitroanilide hydrobromide; Norleucine-4-nitroanilide HBr; 4-Nitroanilide of L-norleucine hydrobromide; L-2-Aminohexanoic acid 4-nitroanilide hydrobromide; Substrate for Leucine Aminopeptidase; LAP Substrate; (S)-2-Aminohexanamide, N-(4-nitrophenyl)-, hydrobromide
EINECS	Contact for details

Quality Control

Our L-Norleucine-4-Nitroanilide Hydrobromide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity and identity confirmation to ensure it meets the exacting standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and reliability for your critical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive; prolonged exposure to light should be avoided to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.