Description

trans-L-4-Hydroxyproline 4-Nitroanilide Hydrochloride is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of specific protease activities, offering high sensitivity and selectivity. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions engaged in proteomics and enzyme kinetics studies.

Application

• Protease Activity Assays: As a chromogenic substrate for the specific detection and kinetic analysis of prolyl endopeptidases and related enzymes.
• Biochemical Research: Used in the study of enzyme mechanisms, inhibition profiling, and substrate specificity for proteolytic enzymes.
• Diagnostic Development: A key component in the formulation of diagnostic kits and reagents for clinical and research applications targeting specific protease biomarkers.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Quality Control in Biologics: Used to monitor and ensure the absence or activity level of specific proteases during the manufacturing of biopharmaceuticals.

Basic Information

Product Name	trans-L-4-Hydroxyproline 4-Nitroanilide Hydrochloride
CAS No.	213271-05-7
Molecular Formula	C16H20N4O5 • HCl
Molecular Weight	408.82 g/mol
Synonyms	H-Hyp-pNA • HCl; L-4-Hydroxyproline 4-nitroanilide hydrochloride; trans-4-Hydroxy-L-proline 4-nitroanilide hydrochloride; H-Hyp-p-nitroanilide hydrochloride; Prolyl endopeptidase substrate; 4-Nitroanilide derivative of trans-4-hydroxy-L-proline hydrochloride; H-Hyp-4-NA • HCl
EINECS	Contact for details

Quality Control

Every batch of trans-L-4-Hydroxyproline 4-Nitroanilide Hydrochloride is subjected to rigorous analytical testing to ensure identity, purity, and performance consistency suitable for sensitive biochemical applications. Our quality system adheres to in-house specifications developed for research-grade chemicals. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	Off-white to yellow crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, yellow solution in DMSO or DMF
Functional Performance	Suitable for enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.