Description

Heptaoxatricosanedioic Acid Bis(n-Hydroxysuccinimide) Ester is a specialized heterobifunctional crosslinking reagent featuring a long, hydrophilic PEG-like spacer arm terminated with two NHS ester groups. This compound is engineered for the efficient and stable conjugation of amine-containing molecules, such as proteins, peptides, or antibodies, to other functional groups or surfaces. It is particularly valuable in life sciences research and bioconjugate development, where controlled and site-specific coupling is required. The product is supplied under strict quality control to ensure batch-to-batch consistency for critical applications.

Application

• Bioconjugate Chemistry: Primary reagent for crosslinking amines to create protein-protein, protein-peptide, or antibody-drug conjugates (ADCs).
• Biomaterial Functionalization: Surface modification of nanoparticles, microspheres, hydrogels, and biosensor chips with bioactive ligands.
• Immunoassay Development: Conjugation of enzymes (e.g., HRP, ALP) or fluorescent dyes to antibodies for ELISA, lateral flow, and other diagnostic platforms.
• Affinity Chromatography: Immobilization of antibodies, proteins, or other affinity ligands onto solid supports like agarose or magnetic beads.
• Drug Delivery Systems: Linking therapeutic payloads to carrier molecules like polymers, lipids, or targeting antibodies.
• Structural Biology & Proteomics: Tool for studying protein-protein interactions and mapping molecular interfaces through crosslinking mass spectrometry (XL-MS).

Basic Information

Product Name	Heptaoxatricosanedioic Acid Bis(n-Hydroxysuccinimide) Ester
CAS No.	211746-85-9
Molecular Formula	C27H40N2O17
Molecular Weight	664.62 g/mol
Synonyms	Bis(NHS)PEG9; Bis(succinimidyl) nonaethyleneglycol dicarboxylate; PEG9 bis(NHS ester); NHS-PEG9-NHS; α,ω-Bis-[N-succinimidyloxycarbonyl]-nonaethyleneglycol; Bis-NHS ester of PEG9 diacid; Nonakis(ethylene glycol) bis(succinimidyl succinate); Heterobifunctional PEG crosslinker, NHS ester terminated
EINECS	Contact for details

Quality Control

Our Heptaoxatricosanedioic Acid Bis(n-Hydroxysuccinimide) Ester is manufactured under a cGMP-compliant quality management system to ensure the highest standards of purity and performance. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR). We provide comprehensive Certificates of Analysis (COA) with detailed specifications for critical parameters such as assay, moisture content (KF), and residual solvents. Our quality commitment supports applications in regulated research and development environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) due to its hygroscopic and moisture-sensitive nature. For long-term stability, store under inert atmosphere (argon or nitrogen). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.