Description

4-Methylumbelliferyl 2-Acetamido-2-Deoxy-3-o-(B-D-Galactopyranosyl)-A-D-Galactopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of specific glycosidase enzyme activity. This compound matters because it provides a reliable, high-throughput method for biochemical research and diagnostic applications, offering excellent sensitivity and a clear fluorescent signal upon enzymatic cleavage. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for studying carbohydrate metabolism and developing enzyme-based assays.

Application

• Enzymology Research: A critical substrate for the specific detection and kinetic study of α-N-acetylgalactosaminidase and related glycosidase enzymes.
• Diagnostic Assay Development: Used in the formulation of fluorogenic kits for the diagnosis of genetic disorders related to lysosomal storage diseases, such as Schindler disease.
• High-Throughput Screening (HTS): Employed in pharmaceutical research to screen for potential enzyme inhibitors or activators in drug discovery pipelines.
• Biomarker Detection: Facilitates the measurement of specific enzyme activities in cell lysates, tissue samples, and bodily fluids as biochemical markers.
• Quality Control in Biologics: Used to monitor and ensure the consistency of enzyme activities in biomanufacturing processes.
• Microbiology Studies: Applied to characterize bacterial glycosidases for microbial identification and functional genomics.

Basic Information

Product Name	4-Methylumbelliferyl 2-Acetamido-2-Deoxy-3-o-(B-D-Galactopyranosyl)-A-D-Galactopyranoside
CAS No.	210357-36-1
Molecular Formula	C24H30N2O14
Molecular Weight	570.50 g/mol
Synonyms	4-MU-α-GalNAc; 4-Methylumbelliferyl α-N-acetylgalactosaminide; 4-MU-α-D-GalNAc; 4-Methylumbelliferyl N-acetyl-α-D-galactosaminide; α-N-Acetylgalactosaminidase Substrate; Fluorogenic α-GalNAc substrate; 4-MU-α-GalNAc (Schindler disease substrate); 2-Acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside 4-methylumbelliferyl
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl glycoside substrates are produced under stringent quality management systems to ensure batch-to-batch consistency and high purity, essential for reproducible research and diagnostic results. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and performance in standard assay conditions. We adhere to relevant guidelines for biochemical reagents to support our global clientele in pharmaceutical, diagnostic, and academic research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Optical Rotation	Contact for details
Fluorogenic Activity	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.