Description

4-Nitrophenyl Phosphate, Potassium Salt is a key chromogenic substrate widely used in enzymatic assays. It serves as a critical reagent for the detection and quantification of alkaline phosphatase (ALP) activity, a common marker in diagnostic and research applications. This high-purity compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and clinical diagnostics industries who require reliable and sensitive assay components.

Application

• Enzymatic Assay Substrate: Primary substrate for colorimetric detection of alkaline phosphatase (ALP) activity in ELISA, Western blotting, and other immunoassays.
• Clinical Diagnostics: Used in automated clinical chemistry analyzers for measuring ALP levels in serum, a key indicator for liver and bone disorders.
• Molecular Biology Research: Essential reagent in nucleic acid detection kits (e.g., Southern, Northern, and Western blots) utilizing ALP-conjugated probes.
• Pharmaceutical Quality Control: Employed in potency testing and quality control of biopharmaceuticals where ALP is used as a reporter enzyme.
• Food Safety Testing: Component in rapid test kits for the detection of pathogenic bacteria, utilizing ALP as an enzymatic label.
• Biochemical Research: Used in kinetic studies and enzyme characterization to determine kinetic parameters (Km, Vmax) of phosphatases.

Basic Information

Product Name	4-Nitrophenyl Phosphate, Potassium Salt
CAS No.	208651-58-5
Molecular Formula	C6H4KNO6P
Molecular Weight	256.17 g/mol
Synonyms	p-Nitrophenyl Phosphate, Potassium Salt; PNPP, K Salt; 4-NPP; Phosphoric Acid 4-Nitrophenyl Ester Potassium Salt; pNPP; Alkaline Phosphatase Substrate; PNPP-K; 4-Nitrophenyl Dihydrogen Phosphate Potassium Salt
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Phosphate, Potassium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is tested to meet stringent specifications for purity, identity, and suitability for use as an enzymatic substrate. A comprehensive Certificate of Analysis (COA) detailing assay purity, impurity profiles, and functional performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive) and should be kept in a desiccated environment to maintain stability and performance. For long-term storage, consider storing at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility (1% in Water)	Clear, colorless to faint yellow solution
Functional Test (ALP Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.