Description

H-Gly-Gly-Amc is a specialized peptide derivative containing a C-terminal 7-amino-4-methylcoumarin (AMC) fluorophore. This compound serves as a critical fluorogenic substrate for the study and detection of protease enzyme activity, particularly dipeptidyl peptidases. It is essential for researchers and manufacturers in the life sciences sector who require high-purity, reliable biochemical tools for diagnostic development, enzymatic assay kits, and fundamental biochemical research. The product is supplied as a high-purity standard to ensure consistent and reproducible experimental results.

Application

• Protease Activity Assays: Primary substrate for fluorometrically monitoring the activity of dipeptidyl peptidases (DPPs) and other aminopeptidases.
• Diagnostic Kit Development: Key component in the formulation of commercial and research-use-only (RUO) diagnostic kits for enzyme-linked diseases.
• High-Throughput Screening (HTS): Used in pharmaceutical research for screening potential inhibitors of target proteases in drug discovery pipelines.
• Biochemical Research: Fundamental tool for studying enzyme kinetics, specificity, and mechanism of action in academic and industrial laboratories.
• Quality Control in Biomanufacturing: Employed to assess the presence and activity of specific proteases in biopharmaceutical production processes.

Basic Information

Product Name	H-Gly-Gly-Amc
CAS No.	208645-74-3
Molecular Formula	C₁₄H₁₆N₄O₄
Molecular Weight	304.30 g/mol
Synonyms	Gly-Gly-AMC; H-Gly-Gly-7-amido-4-methylcoumarin; Glycyl-glycine 7-amido-4-methylcoumarin; DPP Substrate; Dipeptidyl Peptidase Substrate Gly-Gly-AMC; Fluorogenic Peptide Substrate; 7-Amido-4-methylcoumarin conjugate of Gly-Gly; H-Gly-Gly-NH-Mec
EINECS	Contact for details

Quality Control

Every batch of H-Gly-Gly-Amc is manufactured and analyzed under strict quality management protocols to ensure identity, purity, and performance consistency. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request, providing detailed results for purity (HPLC), identity (IR, MS), and other critical parameters.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.