Description

Suc-Tyr-Val-Ala-Asp-Pna is a chromogenic peptide substrate designed for the specific detection and quantification of caspase activity, particularly caspase-3 and related enzymes. This compound matters as a critical research tool for studying apoptosis (programmed cell death) in biochemical and cell-based assays. Researchers and scientists in the fields of oncology, neurobiology, immunology, and drug discovery need it to screen for potential therapeutic compounds and investigate disease mechanisms.

Application

• Caspase-3 Activity Assays: Primary substrate for fluorometric or colorimetric detection of caspase-3 enzyme activity in cell lysates and purified systems.
• Apoptosis Research: Essential tool for quantifying the induction and progression of programmed cell death in various cell lines and tissue samples.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel pro-apoptotic or anti-apoptotic compounds targeting caspase pathways.
• Biochemical Characterization: Employed in enzyme kinetics studies to determine the catalytic efficiency (Km, Vmax) and inhibition constants (Ki) of caspase-3 and related proteases.
• Diagnostic Development: Serves as a key component in developing diagnostic kits for conditions involving dysregulated apoptosis, such as certain cancers and neurodegenerative diseases.
• Toxicology Studies: Applied to assess the apoptotic effects of chemicals, environmental toxins, or new drug candidates on cellular health.

Basic Information

Product Name	Suc-Tyr-Val-Ala-Asp-Pna
CAS No.	208264-84-0
Molecular Formula	C34H43N7O12
Molecular Weight	741.75 g/mol
Synonyms	Ac-YVAD-pNA; Suc-YVAD-pNA; N-Acetyl-Tyr-Val-Ala-Asp p-Nitroanilide; Caspase-1 Substrate (Ac-YVAD-pNA); Caspase-1/ICE Colorimetric Substrate; N-Acetyl-Tyr-Val-Ala-Asp pNA; ICE Substrate II; Apoptosis Detection Substrate; Suc-Tyr-Val-Ala-Asp-p-Nitroanilide
EINECS	Contact for details

Quality Control

Our Suc-Tyr-Val-Ala-Asp-Pna is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, assay results, and solvent content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO or DMF
Functional Activity	Conforms to reference standard in caspase-1 assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.