Description

2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid is a sophisticated bifunctional photoreactive crosslinking agent, expertly engineered for advanced biochemical research and proteomic applications. Its core value lies in the strategic combination of a biotin affinity tag and a photoactivatable diazirine group, enabling the capture and identification of transient or weak protein-protein interactions. This compound is essential for researchers and developers in pharmaceutical discovery, structural biology, and diagnostic assay development who require high-purity, reliable tools for mapping complex biomolecular networks.

Application

• Photoaffinity Labeling (PAL): A critical tool for identifying and characterizing small molecule-protein binding sites and transient interactions in drug discovery.
• Crosslinking Mass Spectrometry (XL-MS): Used to stabilize protein complexes for structural analysis, providing insights into protein architecture and dynamics.
• Biotin-Streptavidin Pull-Down Assays: The biotin moiety allows for efficient isolation and purification of crosslinked protein complexes or labeled targets from complex biological mixtures.
• Proteomics and Interactome Mapping: Employed to discover novel protein-protein interactions and map signaling pathways within cells.
• Development of Diagnostic Kits: Serves as a key reagent in creating assays that detect specific biomolecular interactions for research or clinical use.
• Surface Immobilization Studies: Facilitates the study of biomolecules on surfaces for biosensor development and material science applications.

Basic Information

Product Name	2-[2-[2-[2-[6-(Biotinylaminohexanoyl]Aminoethoxy]Ethoxy]Ethoxy]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoic Acid
CAS No.	207971-23-1
Molecular Formula	C₃₃H₄₆F₃N₅O₈S
Molecular Weight	729.81 g/mol
Synonyms	Biotin-PEG4-Benzophenone (common trade name analog); 4-[3-(Trifluoromethyl)-3H-diazirin-3-yl]benzoic acid-PEG4-Biotin; Biotinylated Photoaffinity Probe; Biotin-PEG4-TFPB; Photoreactive Biotin Crosslinker; Diazirine-PEG4-Biotin; N-(6-(Biotinamido)hexanoyl)-N'-(2-{2-[2-(2-{4-[3-(trifluoromethyl)-3H-diazirin-3-yl]benzamido}ethoxy)ethoxy]ethoxy}ethyl)glycinamide; Biotinylated Diazirine Crosslinker
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical reagent adheres to stringent quality protocols to ensure batch-to-batch consistency and optimal performance. Each lot is characterized using advanced analytical techniques, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment, supporting research that demands the highest levels of reliability and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.