Description

6-Fluoro-3-Indolyl-B-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate used in enzymatic assays. This compound is critically important for the detection and quantification of β-galactosidase activity, a common reporter enzyme in molecular biology and genetic research. It is primarily needed by biotechnology firms, pharmaceutical research and development laboratories, and academic institutions conducting high-throughput screening, gene expression studies, and cell-based assays.

Application

• β-Galactosidase Reporter Gene Assays: Used as a substrate to detect and measure β-galactosidase activity in cell lysates or in situ, commonly in lacZ gene expression studies.
• High-Throughput Screening (HTS): Employed in automated screening platforms within pharmaceutical and biotech R&D to identify enzyme inhibitors or activators.
• Microbiology and Cell Biology Research: Facilitates the study of gene regulation, promoter activity, and cellular differentiation processes in bacterial and eukaryotic cells.
• Diagnostic Kit Development: Serves as a key component in the formulation of diagnostic kits for enzymatic activity detection.
• Molecular Cloning and Genetic Engineering: Acts as a selective and visual marker in blue-white screening protocols for recombinant DNA identification.
• Biochemical Research: Used for kinetic studies and characterization of β-galactosidase enzymes from various sources.

Basic Information

Product Name	6-Fluoro-3-Indolyl-B-D-Galactopyranoside
CAS No.	207727-11-5
Molecular Formula	C20H24FNO11
Molecular Weight	473.41 g/mol
Synonyms	6-Fluoro-3-indolyl β-D-galactopyranoside; 6-Fluoro-3-indolyl-β-D-galactoside; 6-F-3-I-β-D-Gal; 6-Fluoroindol-3-yl β-D-galactopyranoside; 5-Fluoro-3-(β-D-galactopyranosyloxy)-1H-indole; Fluorogenic β-Galactosidase Substrate; X-Gal Fluorinated Analog; 6-Fluoro-3-Indolyl-B-D-Gal
EINECS	Contact for details

Quality Control

Our 6-Fluoro-3-Indolyl-B-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.