Description

L-Alanine-5-Bromo-4-Chloro-3-Indoxyl Ester, Trifluoroacetate Salt is a specialized chromogenic substrate used as a critical reagent in enzymatic detection and histochemical staining. Its value lies in its ability to produce an insoluble, intensely colored indigo dye upon enzymatic cleavage, providing high-contrast visualization for research and diagnostics. This compound is primarily needed by manufacturers of diagnostic kits and research laboratories in the life sciences, particularly for detecting specific bacterial enzymes like β-galactosidase and for advanced cell and tissue staining applications. The product is supplied as the trifluoroacetate salt to enhance stability and solubility.

Application

• Chromogenic Substrate for Diagnostic Assays: A key component in culture media and test strips for the specific detection and identification of microorganisms, such as E. coli and other coliforms, based on β-galactosidase activity.
• Histochemistry and Cytochemistry: Used in laboratory research for the localization and visualization of enzyme activity within tissue sections and cell cultures, providing precise spatial information.
• Molecular Biology Research: Employed as a reporter substrate in cloning (e.g., blue-white screening) and gene expression studies to indicate the presence of functional lacZ gene products.
• Food and Water Safety Testing: Integral to rapid microbiological methods for the enumeration and confirmation of indicator organisms in food, beverage, and water quality control.
• Pharmaceutical Microbiology: Used in sterility testing and microbial limit tests for pharmaceutical products to detect specific contaminating bacteria.
• Development of In-Vitro Diagnostics (IVD): Serves as a raw material for formulating specialized reagents in clinical microbiology and automated diagnostic systems.

Basic Information

Product Name	L-Alanine-5-Bromo-4-Chloro-3-Indoxyl Ester, Trifluoroacetate Salt
CAS No.	207725-18-6
Molecular Formula	C14H13BrClF3N2O5
Molecular Weight	461.61 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, L-Alanine ester, TFA salt; BCIP, L-Alanine ester derivative; 5-Bromo-4-chloro-3-indoxyl-β-D-galactopyranoside alanine ester trifluoroacetate; X-Gal Alanine Ester TFA; Chromogenic Galactosidase Substrate (Alanine ester); 3-Indolyl-β-D-galactopyranoside, 5-bromo-4-chloro-, alanine ester, trifluoroacetate; BCIP-Ala-O-TFA
EINECS	Contact for details

Quality Control

Every batch of L-Alanine-5-Bromo-4-Chloro-3-Indoxyl Ester, Trifluoroacetate Salt is manufactured and tested under a strict quality management system. Our products undergo rigorous quality testing, including advanced chromatographic methods, to ensure high purity, batch-to-batch consistency, and performance reliability for sensitive diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided with each shipment and are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility Test	Clear, colorless solution in DMSO
Functional Performance	Yields intense blue precipitate with β-galactosidase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.