Description

5-Bromo-4-Chloro-3-Indoxyl-β-D-Xylopyranoside is a specialized chromogenic substrate used for the detection and quantification of specific enzymatic activity. This compound is critical for researchers and quality control labs requiring precise, colorimetric assays. It is primarily utilized in the life sciences, diagnostic manufacturing, and biochemical research sectors for applications such as enzyme histochemistry and molecular biology.

Application

• Enzyme Histochemistry: As a chromogenic substrate for the specific detection of β-D-xylosidase activity in tissue sections and cell cultures.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits and reagents for clinical and research laboratories.
• Biochemical Research: Used in studies of glycosidase enzymes, including kinetic analysis, inhibition studies, and enzyme characterization.
• Microbiology: Employed in microbial identification systems to detect xylosidase-producing microorganisms.
• Plant Biology Research: Applied in the study of plant cell wall metabolism and the activity of hemicellulose-degrading enzymes.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to screen for modulators of glycosidase activity.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl-β-D-Xylopyranoside
CAS No.	207606-55-1
Molecular Formula	C13H13BrClNO6
Molecular Weight	394.60 g/mol
Synonyms	X-β-D-Xylopyranoside, 5-Bromo-4-chloro-3-indolyl; 5-Bromo-4-chloro-3-indoxyl β-D-xyloside; BCIP-X; Xyl-β-D-5-Br-4-Cl-3-indolyl; 3-Indoxyl-β-D-xylopyranoside, 5-bromo-4-chloro-; 5-Br-4-Cl-3-indolyl-β-D-xylopyranoside
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl-β-D-Xylopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.