Description

Caged D-Erythro-Sphingosine-1-Phosphate CAS NO 207516-11-8 is a specialized, photolabile derivative of the key bioactive lipid signaling molecule Sphingosine-1-Phosphate (S1P). This compound is engineered for precise, spatiotemporal control in biological research, allowing for the light-triggered release of active S1P. It is an essential tool for researchers in cell biology, neuroscience, and immunology who require high temporal precision to study S1P receptor signaling pathways, cell migration, and vascular development without the confounding effects of traditional pharmacological agents.

Application

• Photochemical Uncaging Studies: Primary tool for investigating the real-time dynamics of S1P signaling upon UV light activation in live cells and tissues.
• S1P Receptor (S1PR) Research: Used to precisely activate S1P1, S1P2, S1P3, S1P4, and S1P5 receptors in a controlled manner to study downstream effects.
• Cell Migration & Chemotaxis Assays: Enables the study of endothelial cell, immune cell (e.g., lymphocyte), and stem cell migration with high spatial and temporal resolution.
• Angiogenesis and Vascular Biology: Critical for models studying blood vessel formation and barrier function where timed S1P release is required.
• Neuroscience Research: Applied in studies of neural development, gliogenesis, and the role of S1P in the central nervous system.
• High-Content/High-Throughput Screening (HCS/HTS): Serves as a precise agonist in screening platforms for drug discovery targeting the S1P pathway.
• Developmental Biology: Used in model organisms (e.g., zebrafish, Xenopus) to map the role of S1P gradients in embryogenesis.
• Signal Transduction Pathway Analysis: Facilitates the dissection of complex intracellular signaling networks activated by S1P receptors.

Basic Information

Product Name	Caged D-Erythro-Sphingosine-1-Phosphate
CAS No.	207516-11-8
Molecular Formula	C29H40NO7P
Molecular Weight	545.60 g/mol
Synonyms	1,2-O-Di-(2-nitrobenzyl)-sn-glycero-3-phosphorylethanolamine; Caged S1P; Photocaged Sphingosine-1-Phosphate; (2S,3R,4E)-2-Amino-4-octadecene-1,3-diol 1-(2-Nitrophenyl)ethyl Phosphate; D-erythro-Sphingosine 1-Phosphate, 1-(2-Nitrophenyl)ethyl Ester; S1P Cage; NB-caged S1P
EINECS	Contact for details

Quality Control

Our Caged D-Erythro-Sphingosine-1-Phosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high chemical purity, essential for reproducible photouncaging experiments. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and stability data. We adhere to rigorous analytical standards suitable for life science research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term storage, keep under an inert atmosphere (argon or nitrogen) to maintain stability. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Assay (NMR)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, ethanol, DMF
Extinction Coefficient (ε)	~5,000 M-1cm-1 at ~260 nm (for uncaging)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.