Description

Hippuryl-His-Leu Free Base is a synthetic tripeptide derivative widely utilized as a key biochemical reagent. This compound is primarily valued for its role as a specific and sensitive substrate for angiotensin-converting enzyme (ACE) activity assays. It is an essential tool for researchers and quality control laboratories in the pharmaceutical and life sciences sectors. The product is supplied as a high-purity free base to ensure optimal performance and stability in critical applications.

Application

• ACE Activity Assays: Primary substrate for the spectrophotometric or fluorometric determination of angiotensin-converting enzyme activity in research and diagnostic settings.
• Pharmaceutical R&D: Used in the screening and development of novel ACE inhibitor drugs for hypertension and heart failure.
• Clinical Diagnostics: Employed in diagnostic kits to measure ACE levels in human serum, aiding in the diagnosis and monitoring of sarcoidosis and other conditions.
• Enzyme Kinetics Studies: A critical reagent for studying the catalytic mechanism, inhibition constants (Ki), and substrate specificity of ACE.
• Biochemical Research: Serves as a model peptide substrate in studies of protease specificity and peptide metabolism.
• Quality Control: Used as a reference standard in the quality assurance of pharmaceutical products targeting the renin-angiotensin system.

Basic Information

Product Name	Hippuryl-His-Leu Free Base
CAS No.	207386-83-2
Molecular Formula	C24H30N6O6
Molecular Weight	498.53 g/mol
Synonyms	Hippuryl-L-Histidyl-L-Leucine; N-Benzoylglycyl-L-histidyl-L-leucine; Bz-Gly-His-Leu; ACE Substrate; Angiotensin Converting Enzyme Substrate; HHL; L-Histidyl-L-leucine, N2-[N-(benzoylamino)acetyl]-; Nα-Hippuryl-L-histidyl-L-leucine
EINECS	Contact for details

Quality Control

Our Hippuryl-His-Leu Free Base is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identification by spectroscopic methods, purity assessment by HPLC, and verification of biological activity. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Biological Activity (ACE Assay)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.