Description

5'-o-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-2'-O,4'-C-Methyleneguanosine is a sophisticated, high-purity nucleoside phosphoramidite derivative, specifically engineered for solid-phase oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of complex nucleic acid constructs. It is an essential raw material for research institutions, diagnostic developers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other advanced therapeutic modalities.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer for the automated synthesis of RNA and modified DNA strands.
• Therapeutic Oligonucleotide Development: Used in the research and GMP-scale production of antisense drugs, siRNA therapeutics, and aptamers targeting various diseases.
• Diagnostic Probe Manufacturing: Essential for creating high-specificity fluorescent probes, molecular beacons, and primers used in PCR, qPCR, and next-generation sequencing (NGS) applications.
• Structural Biology & Biochemistry Research: Enables the study of RNA structure, function, and protein-nucleic acid interactions by incorporating modified guanosine residues.
• Synthesis of Locked Nucleic Acids (LNAs): The 2'-O,4'-C-methylene bridge is a defining feature for creating LNA monomers, which confer enhanced binding affinity and nuclease resistance to oligonucleotides.
• CRISPR/Cas9 Guide RNA Synthesis: Used in the production of chemically modified guide RNAs (gRNAs) to improve stability and editing efficiency.

Basic Information

Product Name	5'-o-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-2'-O,4'-C-Methyleneguanosine
CAS No.	206055-72-3
Molecular Formula	C41H45N5O8
Molecular Weight	735.83 g/mol
Synonyms	5'-O-DMT-N2-Ibu-2'-O,4'-C-methylene Guanosine; 5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-O,4'-C-methyleneguanosine; DMT-N2-Ibu-G(m) Phosphoramidite precursor; 2'-O,4'-C-Methylene Guanosine DMT-protected derivative; LNA-Guanosine (DMT, N2-Ibu) monomer; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-O,4'-C-methyleneguanosine; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O,4'-C-methylene-, 2'-(2-methylpropanoyl) deriv.
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality-controlled conditions to ensure batch-to-batch consistency and purity suitable for sensitive oligonucleotide synthesis. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity (typically ≥98% by HPLC), moisture content, and related substances is provided. Our quality system supports development under GMP guidelines for therapeutic applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.