Description

4,5-Diaminofluorescein Diacetate is a cell-permeable, non-fluorescent precursor dye used extensively as a reactive probe in biochemical research. Its core value lies in its enzymatic conversion to a highly fluorescent product, making it a critical tool for detecting and quantifying specific biological activities. This compound is essential for researchers and manufacturers in the fields of molecular biology, diagnostics, and pharmaceutical development, particularly for assays involving oxidative stress and enzyme activity.

Application

• Fluorescent Probe for Reactive Oxygen Species (ROS) Detection: Primarily used to detect intracellular hydrogen peroxide (H₂O₂) and other peroxides in live-cell imaging and flow cytometry.
• Enzyme Activity Assays: Serves as a substrate for peroxidases (e.g., horseradish peroxidase) in enzymatic assays to study kinetics and inhibition.
• Oxidative Stress Research: A key reagent for monitoring redox states and oxidative damage in cellular models of disease, aging, and toxicity.
• Diagnostic Kit Component: Incorporated into commercial and research-grade diagnostic kits for measuring peroxidase activity or oxidative biomarkers.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify novel antioxidants or modulators of oxidative pathways.
• Neuroscience Research: Applied in studies of neuronal oxidative metabolism and neuroinflammatory responses.

Basic Information

Product Name	4,5-Diaminofluorescein Diacetate
CAS No.	205391-02-2
Molecular Formula	C₂₄H₁₈N₂O₈
Molecular Weight	462.41 g/mol
Synonyms	DAF-2 DA; DAF-2 Diacetate; 4,5-Diamino-2',7'-difluorofluorescein Diacetate; 2',7'-Difluoro-4,5-diaminofluorescein Diacetate; 6-(2,7-Difluoro-3,6-dihydroxy-9H-xanthen-9-yl)-3,4-diaminobenzoic Acid Diacetate; H₂DCFDA (common related probe); Fluorescent ROS Indicator; Reactive Oxygen Species Sensor
EINECS	Contact for details

Quality Control

Our 4,5-Diaminofluorescein Diacetate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Fluorescence Performance	Conforms to functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.