Description

Ac-Tbu-Gly-Tbu-Gly-Asn(Me)2-Ala-Amc is a specialized, protected peptide sequence containing the fluorogenic leaving group 7-amino-4-methylcoumarin (AMC). This compound matters as a critical substrate and intermediate in biochemical research, particularly for studying protease activity and enzyme kinetics. Pharmaceutical R&D laboratories and biotechnology companies need this high-purity peptide for developing and validating sensitive fluorometric assays.

Application

• Protease Substrate: Used as a fluorogenic substrate for the study of specific proteolytic enzymes, where cleavage releases the AMC group, generating a measurable fluorescent signal.
• Enzyme Kinetics & Inhibition Studies: Essential for determining kinetic parameters (Km, Vmax) and screening for potential protease inhibitors in drug discovery pipelines.
• Biochemical Assay Development: Serves as a key component in developing high-throughput screening (HTS) assays for target validation in academic and industrial research.
• Diagnostic Reagent Intermediate: Employed in the synthesis of reagents for clinical diagnostic kits that measure specific protease levels.
• Cell Biology Research: Utilized in live-cell imaging and activity-based protein profiling (ABPP) to visualize and quantify protease activity within cellular environments.

Basic Information

Product Name	Ac-Tbu-Gly-Tbu-Gly-Asn(Me)2-Ala-Amc
CAS No.	204909-38-6
Molecular Formula	C31H48N8O9
Molecular Weight	676.77 g/mol
Synonyms	Acetyl-tert-butylglycyl-tert-butylglycyl-N,N-dimethylasparaginyl-alanyl-7-amino-4-methylcoumarin; N-Acetyl-L-α-tert-butylglycyl-L-α-tert-butylglycyl-N4,N4-dimethyl-L-asparaginyl-L-alanyl-7-amino-4-methylcoumarin; Ac-Tle-Gly-Tle-Gly-Asn(Me)2-Ala-AMC; Fluorogenic Peptide Substrate 204909-38-6; Protease Substrate Ac-Tbu-Gly-Tbu-Gly-Asn(Me)2-Ala-Amc; AMC-labeled Peptide; Ac-Tle-Gly-Tle-Gly-Asn(Me)2-Ala-AMC
EINECS	Contact for details

Quality Control

Our Ac-Tbu-Gly-Tbu-Gly-Asn(Me)2-Ala-Amc is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.