Description

Z-Gly-Ala-Gln-Amc CAS NO 201928-35-0 is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of enzymatic activity. It is primarily utilized by researchers and quality control laboratories in the life sciences, pharmaceutical, and biotechnology industries for high-throughput screening and mechanistic studies.

Application

• Protease Enzyme Assays: As a fluorogenic substrate for measuring the activity of specific proteases, including caspases and other endopeptidases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential protease inhibitors or activators.
• Biochemical Research: A key tool for studying enzyme kinetics, substrate specificity, and catalytic mechanisms in vitro.
• Apoptosis (Programmed Cell Death) Studies: Employed to detect and quantify caspase activity, a central mediator in apoptotic pathways.
• Diagnostic Kit Development: Serves as a core component in the development of sensitive diagnostic assays for diseases involving protease dysregulation.
• Quality Control in Biologics Manufacturing: Used to monitor and validate the activity of enzyme-based therapeutics during production.

Basic Information

Product Name	Z-Gly-Ala-Gln-Amc
CAS No.	201928-35-0
Molecular Formula	C24H31N5O8
Molecular Weight	517.53 g/mol
Synonyms	Z-GAQ-Amc; Z-Gly-Ala-Gln-AMC; Benzyloxycarbonyl-Gly-Ala-Gln-7-amido-4-methylcoumarin; Caspase Substrate; Ac-DEVD-AFC (related class); Fluorogenic Peptide Substrate Z-GAQ-AMC; N-CBZ-Glycyl-L-alanyl-L-glutaminyl-7-amido-4-methylcoumarin; L-Glutaminyl-7-amido-4-methylcoumarin, N2-[(benzyloxy)carbonyl]glycyl-L-alanyl-
EINECS	Contact for details

Quality Control

Our Z-Gly-Ala-Gln-Amc is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and MS for identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF
Water Content (KF)	≤ 1.0%
Fluorogenic Activity	Functional in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.