Description

L-Tryptophan 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic peptide substrate and biochemical reagent. This compound is critical for enzymatic activity assays, particularly for proteases like trypsin and chymotrypsin, where it serves as a highly sensitive reporter molecule. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers for high-throughput screening and biochemical analysis.

Application

• Protease Activity Assays: A key fluorogenic substrate for measuring the activity of serine proteases, including trypsin and chymotrypsin, in research and drug discovery.
• High-Throughput Screening (HTS): Used in automated pharmaceutical screening platforms to identify and characterize potential enzyme inhibitors or activators.
• Biochemical Research: Employed in kinetic studies and enzyme mechanism investigations within academic and industrial laboratories.
• Diagnostic Development: Serves as a core component in developing sensitive fluorometric diagnostic tests for enzymatic biomarkers.
• Cell Biology Studies: Applied in assays to detect and quantify protease activity within cellular systems and tissue samples.
• Quality Control in Biomanufacturing: Used to monitor the presence and activity of specific proteases during biopharmaceutical production processes.

Basic Information

Product Name	L-Tryptophan 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	201860-49-3
Molecular Formula	C22H22N4O4 • HCl
Molecular Weight	442.89 g/mol (Free base: 406.44 g/mol)
Synonyms	L-Tryptophan 7-amido-4-methylcoumarin HCl; AMC-Tryptophan; Tryptophan-AMC Hydrochloride; 7-Amido-4-methylcoumarin-L-tryptophan hydrochloride; Boc-L-Trp-AMC HCl; Protease Substrate AMC-Trp; Fluorogenic trypsin substrate; (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-3-(1H-indol-3-yl)propanoic acid
EINECS	Contact for details

Quality Control

Our L-Tryptophan 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and stringent controls for moisture and related substances. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, allow the container to reach room temperature before opening to minimize moisture absorption. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.