Description

H-Lys(Z)-Amc Hcl CAS NO 201853-27-2 is a protected amino acid derivative, specifically an Nα-Carbobenzyloxy (Cbz or Z) protected lysine residue coupled to 7-Amino-4-methylcoumarin (AMC). This compound is a critical fluorogenic substrate and a key synthetic intermediate in peptide chemistry and biochemical research. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for enzyme activity assays, protease research, and the synthesis of complex peptide-based molecules.

Application

• Fluorogenic Protease Substrate: Used in high-throughput screening (HTS) and kinetic studies to measure the activity of various proteolytic enzymes, including trypsin, plasmin, and other serine proteases.
• Peptide Synthesis Intermediate: Serves as a key building block in solid-phase and solution-phase peptide synthesis (SPPS) for incorporating a protected lysine residue with a fluorescent reporter tag.
• Biochemical Research Reagent: Employed in developing sensitive assays for studying enzyme mechanisms, inhibition, and cellular signaling pathways.
• Diagnostic Kit Development: A component in the formulation of diagnostic kits for detecting and quantifying specific protease activities relevant to disease states.
• Pharmaceutical R&D: Used in drug discovery programs targeting protease enzymes, aiding in the identification and optimization of novel therapeutic candidates.

Basic Information

Product Name	H-Lys(Z)-Amc Hcl
CAS No.	201853-27-2
Molecular Formula	C24H28N4O5 • HCl
Molecular Weight	489.97 g/mol (Free base: 452.50 g/mol)
Synonyms	Nα-Cbz-L-lysine 7-amido-4-methylcoumarin hydrochloride; L-Lysine, N2-[(phenylmethoxy)carbonyl]-, 1-(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-yl) ester, hydrochloride; Z-Lys-AMC • HCl; Cbz-L-Lys-AMC hydrochloride; N-Carbobenzyloxy-L-lysine 7-amido-4-methylcoumarin hydrochloride; H-Lys(Z)-AMC hydrochloride; Lys(Z)-AMC • HCl; Fluorogenic substrate for proteases
EINECS	Contact for details

Quality Control

Our H-Lys(Z)-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture content. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.