Description

H-Gly-Phe-Amc is a specialized fluorogenic peptide substrate widely used in biochemical and pharmaceutical research. This compound is critical for the sensitive detection and quantification of protease enzyme activity, particularly for enzymes like dipeptidyl peptidase IV (DPP-IV). It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, high-purity substrates for enzyme kinetics, inhibitor screening, and assay development.

Application

• Protease Activity Assays: Primary substrate for fluorometric assays measuring DPP-IV and related dipeptidyl peptidase activities.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: A key reagent in enzymatic studies to investigate substrate specificity, kinetic parameters (Km, Vmax), and catalytic mechanisms.
• Diagnostic Kit Development: Employed in the formulation of diagnostic kits for clinical research targeting protease-related biomarkers.
• Quality Control (QC) Testing: Used in pharmaceutical QC labs to monitor the activity and consistency of enzyme-based biopharmaceutical products.
• Academic Research: Fundamental tool in university and institutional labs for studying proteolysis, cell signaling pathways, and metabolic disorders.

Basic Information

Product Name	H-Gly-Phe-Amc
CAS No.	201852-70-2
Molecular Formula	C₂₁H₂₂N₄O₄
Molecular Weight	394.43 g/mol
Synonyms	Glycylphenylalanine 7-amido-4-methylcoumarin; Gly-Phe-AMC; H-Gly-Phe-AMC; GLY-PHE-AMC; DPP-IV Substrate; Dipeptidyl Peptidase IV Fluorogenic Substrate; 7-Amido-4-methylcoumarin derivative of Gly-Phe; H-Glycine-L-phenylalanine-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Every batch of H-Gly-Phe-Amc is manufactured and tested under strict quality management protocols to ensure the highest standards of purity and performance. Our products undergo rigorous quality testing, including HPLC for purity and identity confirmation, to ensure compliance with research-grade specifications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your regulatory and quality assurance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.