Description

H-Gly-Ala-Amc Hcl is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like aminopeptidases. It serves as an essential tool in biochemical research, pharmaceutical development, and diagnostic applications where accurate detection of enzymatic cleavage is paramount.

Application

• Protease Activity Assays: Primary substrate for the fluorometric detection and quantification of specific aminopeptidase and protease activities in research settings.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential enzyme inhibitors or activators.
• Biochemical Research: A key reagent for studying enzyme kinetics, substrate specificity, and catalytic mechanisms of proteolytic enzymes.
• Diagnostic Development: Employed in the development of sensitive diagnostic kits for diseases where specific protease levels are a biomarker.
• Pharmaceutical QC: Utilized in quality control processes to test the purity and activity of enzyme-based pharmaceuticals.
• Cell Biology Studies: Applied in live-cell imaging and assays to monitor protease activity within cellular pathways.

Basic Information

Product Name	H-Gly-Ala-Amc Hcl
CAS No.	201852-69-9
Molecular Formula	C15H19N5O4 • HCl
Molecular Weight	385.81 g/mol
Synonyms	Glycine-Alanine-7-Amino-4-methylcoumarin Hydrochloride; H-Gly-Ala-AMC•HCl; Gly-Ala-AMC substrate; Gly-Ala-AFC (related fluorophore); Aminopeptidase Substrate Gly-Ala-AMC; L-Glycyl-L-alanine 7-amido-4-methylcoumarin hydrochloride; Fluorogenic peptide substrate Gly-Ala-AMC; Protease substrate 201852-69-9
EINECS	Contact for details

Quality Control

Every batch of H-Gly-Ala-Amc Hcl is produced and tested under stringent quality management systems to ensure exceptional purity and performance for critical research applications. Our products undergo rigorous quality testing, including advanced chromatographic analysis, to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and assay results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Fluorogenic Activity	Functional batch testing data provided

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.