Description

Boc-Gln-Ala-Arg-Amc Hcl is a protected tetrapeptide fluorogenic substrate, specifically designed for protease activity assays. This compound is of significant value in biochemical research for its high specificity and sensitivity in detecting and quantifying enzymatic activity. It is primarily needed by researchers and manufacturers in the fields of pharmaceutical development, proteomics, and diagnostic reagent production for studying enzyme kinetics and inhibitor screening.

Application

• Protease Research & Assay Development: Acts as a key fluorogenic substrate for studying the activity and inhibition profiles of specific proteases, including trypsin-like enzymes.
• Pharmaceutical High-Throughput Screening (HTS): Utilized in drug discovery campaigns to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Reagent Manufacturing: Serves as a critical raw material in the production of diagnostic kits and research-grade assay systems for clinical and academic laboratories.
• Enzyme Kinetics & Mechanism Studies: Provides a reliable tool for determining kinetic parameters (Km, Vmax, kcat) and elucidating the catalytic mechanisms of target proteases.
• Quality Control in Biologics: Used in the biopharmaceutical industry to monitor and ensure the consistency and activity of enzyme-based products during manufacturing.

Basic Information

Product Name	Boc-Gln-Ala-Arg-Amc Hcl
CAS No.	201849-55-0
Molecular Formula	C29H45N9O8 • xHCl
Molecular Weight	Contact for details
Synonyms	Boc-Gln-Ala-Arg-AMC hydrochloride; t-Butyloxycarbonyl-Glutaminyl-Alanyl-Arginyl-7-Amino-4-Methylcoumarin hydrochloride; Boc-Gln-Ala-Arg-AMC HCl; Boc-Gln-Ala-Arg-AMC Hcl; Boc-Gln-Ala-Arg-AMC; Fluorogenic Substrate for Protease; t-BOC-Gln-Ala-Arg-7-amido-4-methylcoumarin hydrochloride; Protease Substrate IV (Boc-Gln-Ala-Arg-AMC)
EINECS	Contact for details

Quality Control

Our Boc-Gln-Ala-Arg-Amc Hcl is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.