Description

H-β-Ala-Amc Tfa is a specialized fluorogenic peptide substrate, widely recognized as a key tool compound in biochemical and pharmaceutical research. Its core value lies in its role as a sensitive and specific substrate for various proteolytic enzymes, enabling precise activity measurements and high-throughput screening. This product is essential for researchers and manufacturers in the fields of protease inhibitor development, diagnostic assay formulation, and enzymatic mechanism studies.

Application

• Protease Activity Assays: A primary fluorogenic substrate for measuring the activity of serine and cysteine proteases in kinetic studies.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors.
• Biochemical Research: Critical for studying enzyme kinetics, substrate specificity, and catalytic mechanisms of various proteolytic enzymes.
• Diagnostic Development: Employed in the development of sensitive fluorometric diagnostic kits for detecting specific enzyme activities related to diseases.
• Quality Control in Biologics: Used to monitor and quantify protease activity during the production and purification of biopharmaceuticals.
• Cell Biology Studies: Applied in assays to detect and localize protease activity within live cells or tissue samples.

Basic Information

Item	Detail
Product Name	H-β-Ala-Amc Tfa
CAS No.	201847-54-3
Molecular Formula	C₁₇H₂₀F₃N₃O₆
Molecular Weight	419.36 g/mol
Synonyms	H-β-Ala-AMC TFA; N-(3-Aminopropanoyl)-7-amino-4-methylcoumarin Trifluoroacetate; β-Alanine-7-amido-4-methylcoumarin TFA Salt; H-β-Ala-AMC×TFA; H-β-Ala-AMC trifluoroacetate salt; Substrate for Proteases; Fluorogenic Peptide Substrate β-Ala-AMC TFA
EINECS	Contact for details

Quality Control

Our H-β-Ala-Amc Tfa is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation via spectroscopic methods. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to guarantee compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.