Description

H-Val-Leu-Lys-Pna is a synthetic peptide substrate designed for biochemical and pharmaceutical research. This compound is critical for studying enzyme kinetics and inhibition, particularly for proteases like trypsin and related serine proteases. It is an essential tool for researchers and developers in the fields of pharmaceutical discovery, diagnostic assay development, and biochemical research, where precise substrate specificity is required.

Application

• Enzyme Substrate for Protease Research: Primary substrate for kinetic studies and characterization of trypsin-like serine proteases.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitor drug candidates.
• Diagnostic Reagent Development: A key component in developing colorimetric or fluorometric diagnostic kits for detecting specific protease activity.
• Biochemical Mechanism Studies: Employed to elucidate cleavage specificity and catalytic mechanisms of proteolytic enzymes.
• Quality Control in Biologics: Used to test for the presence and activity of specific proteases in biopharmaceutical manufacturing processes.
• Academic and Industrial R&D: A fundamental reagent in life science research laboratories for studying protein-protein interactions and signaling pathways.

Basic Information

Item	Detail
Product Name	H-Val-Leu-Lys-Pna
CAS No.	201807-16-1
Molecular Formula	C23H37N7O5
Molecular Weight	491.59 g/mol
Synonyms	Val-Leu-Lys-p-nitroanilide; H-Val-Leu-Lys-pNA; VLK-pNA; Tosyl-Gly-Pro-Lys 4-nitroanilide acetate salt (related); Trypsin Substrate V; Boc-Val-Leu-Lys-AMC (fluorogenic analog); Protease Substrate; N-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (BAPNA) analog
EINECS	Contact for details

Quality Control

Our H-Val-Leu-Lys-Pna is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay systems. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards of the research and pharmaceutical industries.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	≥ 90% of reference standard in trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.