Description

H-Gly-Glu-Pna CAS NO 201735-59-3 is a specialized peptide derivative, specifically a chromogenic substrate designed for enzymatic activity studies. This compound is critical for biochemical research and diagnostic assay development, providing a reliable and sensitive means to detect and quantify specific protease activities. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on enzymology and biomarker discovery.

Application

• Biochemical Research: Used as a specific chromogenic substrate for studying the kinetics and inhibition of target proteases, such as dipeptidyl peptidases.
• Diagnostic Assay Development: Serves as a key component in the development of colorimetric or fluorometric diagnostic tests for clinical and research purposes.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel enzyme inhibitors for drug discovery programs.
• Enzyme Characterization: Facilitates the determination of enzyme specificity, optimal pH, and temperature profiles in vitro.
• Quality Control in Biomanufacturing: Can be used to monitor the activity and consistency of enzyme batches during production processes.

Basic Information

Product Name	H-Gly-Glu-Pna
CAS No.	201735-59-3
Molecular Formula	C14H18N4O6
Molecular Weight	338.32 g/mol
Synonyms	Glycyl-glutamyl-p-nitroanilide; H-Gly-Glu-pNA; Gly-Glu-pNA; N-Glycyl-L-glutamic acid 4-nitroanilide; Substrate for DPP-IV; Chromogenic substrate for dipeptidyl peptidase; Peptide p-nitroanilide substrate; L-Glutamic acid, N-(N-glycyl)-, 4-nitroanilide
EINECS	Contact for details

Quality Control

Our H-Gly-Glu-Pna is produced under stringent quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, identity confirmation by spectroscopic methods (IR, NMR), and validation of performance in enzymatic assays. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with research-grade material standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.