Description

Bz-Pro-Phe-Pna is a synthetic peptide derivative, specifically a chromogenic substrate designed for enzymatic studies. This compound is a critical tool for researchers and developers in life sciences, offering high specificity and sensitivity for protease activity assays. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic kit manufacturing for studying enzyme kinetics and inhibitor screening.

Application

• Biochemical Research: As a chromogenic substrate for the study of protease and peptidase enzyme kinetics, mechanism, and inhibition.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and characterize potential therapeutic enzyme inhibitors.
• Diagnostic Development: A key component in the development of colorimetric diagnostic assays and test kits for detecting specific enzymatic activity.
• Enzyme Characterization: Employed to determine the substrate specificity and catalytic efficiency of newly discovered or engineered proteases.
• Quality Control in Bioprocessing: Monitoring protease activity during fermentation and downstream purification processes in biomanufacturing.

Basic Information

Product Name	Bz-Pro-Phe-Pna
CAS No.	201734-25-0
Molecular Formula	C27H26N4O5
Molecular Weight	486.52 g/mol
Synonyms	Benzoyl-Pro-Phe-pNA; Bz-Pro-Phe-p-Nitroanilide; N-Benzoyl-L-prolyl-L-phenylalanyl-p-nitroanilide; Chromogenic Substrate for Proteases; Protease Substrate Bz-Pro-Phe-pNA; L-Phenylalanine, N-[N-(N-benzoyl-L-prolyl)-L-phenylalanyl]-4-nitroanilide; Bz-Pro-Phe-pNA (Protease Substrate); Bz-Pro-Phe-4-NA
EINECS	Contact for details

Quality Control

Our Bz-Pro-Phe-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Our commitment to quality assurance guarantees batch-to-batch consistency for reliable experimental results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.