Description

Boc-Ala-Ala-Asp-Pna is a protected tetrapeptide sequence featuring a para-nitroanilide (Pna) chromogenic group. This compound is a critical substrate for the study and detection of proteolytic enzyme activity, particularly for caspases and other aspartic acid-specific proteases. It is essential for researchers and manufacturers in the fields of biochemical research, pharmaceutical development, and diagnostic assay kits.

Application

• Enzyme Substrate for Caspase Assays: Used as a key chromogenic substrate in colorimetric assays to measure caspase-3, -6, -7, -8, and -9 activity, crucial for apoptosis research.
• Protease Activity Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Biochemical Research Reagent: Serves as a standard and reference material in academic and industrial laboratories studying protease kinetics and specificity.
• Diagnostic Kit Component: Integrated into commercial diagnostic kits for the detection and quantification of protease activity in clinical and research samples.
• Pharmaceutical Development: Used in the development and quality control of therapeutic proteins and biologics where protease activity must be monitored.
• Cell Biology Studies: Applied in cell-based assays to monitor programmed cell death pathways and evaluate the efficacy of chemotherapeutic agents.

Basic Information

Product Name	Boc-Ala-Ala-Asp-Pna
CAS No.	201732-83-4
Molecular Formula	C₂₃H₃₂N₆O₉
Molecular Weight	536.54 g/mol
Synonyms	Boc-AAD-pNA; tert-Butyloxycarbonyl-Ala-Ala-Asp-p-Nitroanilide; Boc-Ala-Ala-Asp-pNA; N-(tert-Butoxycarbonyl)-L-alanyl-L-alanyl-L-α-aspartyl 4-nitroanilide; Ac-DEVD-pNA (contextual analog); Caspase-3 Substrate I (chromogenic); Boc-AADP-pNA; L-Alanine, N-[(1S)-1-[[(2S)-1-[[(1,1-dimethylethoxy)carbonyl]amino]-1-oxo-2-[(4-nitrophenyl)amino]-2-oxoethyl]amino]-3-carboxy-1-oxopropyl]-
EINECS	Contact for details

Quality Control

Our Boc-Ala-Ala-Asp-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and chiral analysis to ensure stereochemical integrity. Certificates of Analysis (COA) detailing all specifications are provided to guarantee consistency and reliability for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.