Description

H-Ala-Phe-Pro-Pna is a specialized tripeptide derivative containing a para-nitroanilide (Pna) chromogenic group. This compound is primarily valued as a high-purity, synthetic substrate for enzymatic activity assays and biochemical research. It is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors who require precise tools for studying protease kinetics and inhibitor screening. The product is supplied as a high-grade material to ensure reliable and reproducible experimental results.

Application

• Protease Substrate: Used as a specific chromogenic substrate for the kinetic analysis and characterization of various proteolytic enzymes.
• Biochemical Research: Employed in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors for drug discovery.
• Enzyme Mechanism Studies: Facilitates the study of enzyme specificity, catalytic efficiency, and reaction mechanisms in academic and industrial R&D laboratories.
• Diagnostic Reagent Manufacturing: Serves as a key intermediate or component in the development of diagnostic kits and reagents for clinical analysis.
• Pharmaceutical Development: Utilized in quality control and potency testing during the development of therapeutic proteins and peptide-based drugs.

Basic Information

Product Name	H-Ala-Phe-Pro-Pna
CAS No.	201732-35-6
Molecular Formula	C23H27N5O5
Molecular Weight	453.49 g/mol
Synonyms	Ala-Phe-Pro-pNA; L-Alanyl-L-phenylalanyl-L-prolyl-4-nitroanilide; AFP-pNA; H-Ala-Phe-Pro-4-nitroanilide; Substrate for DPP IV; Dipeptidyl Peptidase IV Substrate; Ala-Phe-Pro-NA; N-(3-(1-((S)-1-((S)-2-Aminopropanamido)-3-phenylpropanamido)prolyl)phenyl)-4-nitrobenzenesulfonamide (related IUPAC-style name)
EINECS	Contact for details

Quality Control

Our H-Ala-Phe-Pro-Pna is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Single Unknown Impurity (HPLC)	≤ 0.5%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.