Description

H-Ala-Ala-Pro-Pna Hcl is a synthetic peptide derivative, specifically a chromogenic substrate designed for enzymatic activity studies. This compound is of significant value in biochemical and pharmaceutical research for its role in the specific detection and quantification of protease enzyme activity. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in drug discovery and proteomics research.

Application

• Protease Enzyme Assays: Used as a selective chromogenic substrate for kinetic studies and activity measurements of specific proteolytic enzymes.
• Biochemical Research: A critical tool in academic and industrial laboratories for investigating enzyme mechanisms, inhibition, and substrate specificity.
• Diagnostic Development: Employed in the research and development of clinical diagnostic tests that rely on enzymatic detection methods.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitor drug candidates.
• Quality Control in Biomanufacturing: Applied in monitoring the activity of enzyme-based products or processes within biopharmaceutical production.

Basic Information

Product Name	H-Ala-Ala-Pro-Pna Hcl
CAS No.	201732-27-6
Molecular Formula	C17H24N6O4 • HCl
Molecular Weight	404.87 g/mol (Free base: 368.41 g/mol)
Synonyms	Ala-Ala-Pro-p-Nitroanilide hydrochloride; L-Alanyl-L-alanyl-L-prolyl-4-nitroanilide hydrochloride; AAPF-pNA HCl; H-AAP-pNA HCl; Substrate for Leukocyte Elastase; N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide related substrate; Protease Substrate IV; 4-Nitroanilide derivative of Ala-Ala-Pro
EINECS	Contact for details

Quality Control

Our H-Ala-Ala-Pro-Pna Hcl is produced and tested under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	< 20 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.