Description

Chlorophenol Red-β-D-Galactopyranoside is a chromogenic substrate specifically designed for enzymatic assays. This compound is critical for the sensitive and quantitative detection of β-galactosidase activity, a widely used reporter system in molecular biology. It is an essential reagent for researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic industries requiring precise enzyme activity measurements.

Application

• β-Galactosidase Reporter Assays: Primary substrate for detecting and quantifying β-galactosidase expression in molecular biology and genetic engineering.
• Microbial Detection & Identification: Used in culture media and diagnostic kits to identify microorganisms (e.g., E. coli) based on β-galactosidase production.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to screen for modulators of gene expression using β-galactosidase as a reporter.
• Cell-Based Assays: Facilitates the study of promoter activity and gene regulation in live cells via colorimetric readouts.
• Enzyme Kinetics & Characterization: Serves as a specific substrate for determining the kinetic parameters (Km, Vmax) of β-galactosidase enzymes.
• Diagnostic Test Development: A key component in the formulation of clinical and point-of-care diagnostic tests for enzymatic deficiencies or bacterial infections.

Basic Information

Product Name	Chlorophenol Red-β-D-Galactopyranoside
CAS No.	201685-76-9
Molecular Formula	C25H21ClO9S
Molecular Weight	532.95 g/mol
Synonyms	CPRG; Chlorophenol Red-β-D-Galactopyranoside; 3'-Chloro-3''-[(6-O-β-D-galactopyranosyl-β-D-galactopyranosyl)oxy]phenolsulfonephthalein; β-Galactosidase Substrate CPRG; CPRG Substrate; Chlorophenolred-β-D-galactoside; Chromogenic Galactosidase Substrate
EINECS	Contact for details

Quality Control

Every batch of Chlorophenol Red-β-D-Galactopyranoside is manufactured and tested under strict quality management systems. We ensure high purity and consistent performance suitable for sensitive research and diagnostic applications. Comprehensive testing includes identity confirmation, purity assay, and performance validation in enzymatic assays. A Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	Red to reddish-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance Spectrum	Conforms to reference
Enzymatic Activity	Suitable for β-galactosidase detection
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.