Description

Ac-Ala-Gln-Ala-Pna CAS NO 201677-60-3 is a synthetic peptide derivative, specifically an acetylated tetrapeptide para-nitroanilide (Pna) substrate. This compound is a critical biochemical tool used as a chromogenic substrate for the study and quantification of specific protease enzyme activity. It is essential for researchers and manufacturers in the life sciences sector who require precise, high-purity reagents for enzymatic assays, drug discovery, and biochemical research. The product offers reliable performance in kinetic studies and high-throughput screening applications.

Application

• Protease Enzyme Assays: Primary use as a chromogenic substrate for quantifying the activity of specific proteolytic enzymes, such as elastase or related serine proteases.
• Biochemical Research: A key reagent in fundamental research to study enzyme kinetics, inhibition mechanisms, and substrate specificity.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms within drug discovery pipelines to identify and characterize novel protease inhibitors.
• Diagnostic Development: Serves as a component in the development of in-vitro diagnostic kits for detecting enzymatic activity related to certain physiological or pathological conditions.
• Quality Control in Biologics: Employed in QC laboratories to monitor protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Ac-Ala-Gln-Ala-Pna
CAS No.	201677-60-3
Molecular Formula	C₂₂H₃₁N₇O₈
Molecular Weight	521.53 g/mol
Synonyms	Acetyl-Alanyl-Glutaminyl-Alanyl-p-Nitroanilide; N-Acetyl-L-alanyl-L-glutaminyl-L-alanine 4-nitroanilide; Ac-AQA-pNA; Ac-Ala-Gln-Ala-pNA; Substrate for Human Leukocyte Elastase (HLE); Peptide Substrate Suc-AAPF-pNA Analog; Protease Substrate 201677-60-3; N-Acetyl-L-Ala-L-Gln-L-Ala-pNA
EINECS	Contact for details

Quality Control

Every batch of Ac-Ala-Gln-Ala-Pna is produced under stringent quality management protocols. Our products undergo rigorous analytical testing, including HPLC for purity verification and MS for identity confirmation, to ensure compliance with research-grade standards. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.