Description

Phenylthiohydantoin Histidine is a specialized derivative of the amino acid histidine, formed by reaction with phenyl isothiocyanate (PITC) in the Edman degradation process. This compound is critical for the sequential analysis of peptides and proteins, enabling the precise identification of amino acid sequences. It is an essential analytical reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and proteomics sectors, where understanding protein structure is paramount for drug development and biochemical research.

Application

• Edman Degradation Sequencing: Primary reagent for the stepwise N-terminal sequencing of peptides and proteins.
• Proteomics Research: Used in protein characterization, identification, and structural analysis workflows.
• Pharmaceutical Development: Supports the analysis and quality control of peptide-based drug substances and biologics.
• Biochemical Analysis: Employed in academic and industrial laboratories for studying protein function and modifications.
• Amino Acid Analysis: Serves as a reference standard or intermediate in advanced chromatographic identification methods.
• Peptide Synthesis Monitoring: Used to verify sequence fidelity during solid-phase or solution-phase peptide synthesis.

Basic Information

Product Name	Phenylthiohydantoin Histidine
CAS No.	29635-98-1
Molecular Formula	C13H14N4O2S
Molecular Weight	290.34 g/mol
Synonyms	PTH-Histidine; 2-(3-Phenyl-2-thioxo-4-imidazolidinyl)-3-(1H-imidazol-4-yl)propanoic Acid; Histidine Phenylthiohydantoin; Histidine, PTH derivative; 1-(Phenylthiocarbamoyl)histidine; Edman Degradation Product of Histidine; PTC-Histidine Cyclization Product
EINECS	Contact for details

Quality Control

Our Phenylthiohydantoin Histidine is produced and tested under strict quality management systems to ensure high purity and batch-to-batch consistency suitable for sensitive analytical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles, typically determined by HPLC, NMR, and mass spectrometry. We support compliance with relevant research and analytical standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Related Substances (HPLC)	Individual impurity ≤ 0.5%; Total impurities ≤ 1.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.