Description

4-Aminophenyl-1-Thio-β-D-Galactopyranoside is a specialized thiogalactoside derivative, a crucial chromogenic and fluorogenic substrate for the detection of β-galactosidase activity. Its core value lies in its high specificity and sensitivity, enabling precise enzymatic assays in molecular biology and diagnostic applications. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic kit production industries.

Application

• β-Galactosidase Reporter Gene Assays: Primary substrate for detecting and quantifying β-galactosidase expression in molecular biology, used in promoter studies and gene regulation research.
• Diagnostic Reagent Manufacturing: Key component in the formulation of diagnostic kits for microbiology and clinical chemistry to identify enzyme-producing organisms.
• Cell-Based Screening: Utilized in high-throughput screening (HTS) platforms in drug discovery to identify modulators of gene expression linked to the lacZ reporter.
• Enzyme Kinetics Studies: Serves as a specific substrate for studying the kinetics and inhibition of β-galactosidase enzymes in biochemical research.
• Histochemistry & In Situ Staining: Used in histological staining techniques to visualize spatial gene expression patterns in tissues and embryos.
• Microbial Detection: Applied in culture media for the selective identification of E. coli and other β-galactosidase-positive bacteria in food and water safety testing.

Basic Information

Product Name	4-Aminophenyl-1-Thio-β-D-Galactopyranoside
CAS No.	29558-05-2
Molecular Formula	C12H17NO5S
Molecular Weight	287.33 g/mol
Synonyms	4-Aminophenyl 1-Thio-β-D-galactopyranoside; Phenyl-β-D-galactopyranoside, 4-amino-1-thio-; APTG; 4-Amino-1-thiophenyl-β-D-galactopyranoside; 4-APTG; 4-Aminophenyl β-D-thiogalactopyranoside; β-D-Galactopyranoside, 4-aminophenyl 1-thio-; 29558-05-2
EINECS	Contact for details

Quality Control

Our 4-Aminophenyl-1-Thio-β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The compound is light-sensitive; prolonged exposure to light should be avoided to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Test in DMSO or DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.