Description

N(Alpha)-Carbobenzoxyarginine-4-Nitroanilide is a specialized synthetic peptide derivative designed for biochemical research and diagnostic applications. This compound serves as a critical chromogenic substrate for the sensitive detection and kinetic analysis of trypsin-like serine proteases. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent sectors who require high-purity, reliable substrates for enzyme activity assays and inhibitor screening.

Application

• Protease Research: Primary substrate for the spectrophotometric assay of trypsin, thrombin, plasmin, and other arginine-specific serine proteases.
• Diagnostic Reagent Manufacturing: Key component in the formulation of chromogenic test kits for clinical diagnostics, particularly for coagulation and fibrinolysis studies.
• Pharmaceutical Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic applications.
• Enzyme Kinetics: Enables precise measurement of Michaelis-Menten constants (Km) and inhibition constants (Ki) due to its specific cleavage and colorimetric signal generation.
• Biochemical Standard: Acts as a reference standard for calibrating enzymatic activity in quality control laboratories.
• Academic Research: Utilized in university and institutional labs for fundamental studies on protease structure, function, and mechanism.

Basic Information

Product Name	N(Alpha)-Carbobenzoxyarginine-4-Nitroanilide
CAS No.	29542-03-8
Molecular Formula	C20H25N7O6
Molecular Weight	459.46 g/mol
Synonyms	Z-Arg-pNA; Carbobenzoxy-Arg-p-nitroanilide; Cbz-Arg-pNA; Nα-CBZ-L-arginine 4-nitroanilide; Nα-Carbobenzyloxy-L-arginine-p-nitroanilide; Benzyloxycarbonyl-L-arginine 4-nitroanilide; Chromogenic substrate for trypsin; Bz-Arg-pNA (common abbreviation)
EINECS	Contact for details

Quality Control

Our N(Alpha)-Carbobenzoxyarginine-4-Nitroanilide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and verification of chromogenic activity. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry, cool place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Chromogenic Activity	Functional in standard trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.