Description

H-Arg(No2)-Pna . Hbr is a chemically modified arginine derivative, specifically the hydrobromide salt of NG-Nitro-L-arginine p-nitroanilide. This compound is a critical biochemical tool, primarily serving as a chromogenic substrate for the detection and study of nitric oxide synthase (NOS) activity. It is essential for researchers and manufacturers in the life sciences sector who require precise, high-purity reagents for enzymatic assays and diagnostic development. Its reliable performance makes it a cornerstone for investigating nitric oxide pathways in both academic and industrial R&D settings.

Application

• Nitric Oxide Synthase (NOS) Assays: Primary chromogenic substrate for the colorimetric detection and quantification of NOS enzyme activity in vitro.
• Biochemical Research: Used in fundamental research to study the kinetics, inhibition, and regulation of nitric oxide production pathways.
• Diagnostic Kit Development: A key component in the formulation of commercial diagnostic kits and reagents for measuring NOS levels in biological samples.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential NOS inhibitor drug candidates.
• Cell Biology Studies: Applied in cellular assays to monitor real-time nitric oxide signaling and its physiological effects.
• Enzyme Characterization: Utilized for the purification and functional characterization of different NOS isoforms (e.g., nNOS, eNOS, iNOS).

Basic Information

Product Name	H-Arg(No2)-Pna . Hbr
CAS No.	29028-61-3
Molecular Formula	C18H22BrN7O5
Molecular Weight	496.32 g/mol
Synonyms	NG-Nitro-L-arginine p-nitroanilide hydrobromide; L-NG-Nitroarginine 4-nitroanilide HBr; Nω-Nitro-L-arginine p-nitroanilide hydrobromide; H-Arg(NO2)-PNA . HBr; NOS Substrate; Nitric Oxide Synthase Chromogenic Substrate; 4-Nitroanilide of NG-Nitro-L-arginine HBr
EINECS	Contact for details

Quality Control

Our H-Arg(No2)-Pna . Hbr is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive biochemical applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines
Biological Activity	Suitable for NOS assay (reference value provided on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.