Description

2,2'-Azino-Di-(3-Ethylbenzothiazoline)-6-Sulfonic Acid is a high-purity chromogenic substrate widely used in enzymatic assays. This compound matters for its critical role as a sensitive and reliable reagent in diagnostic and research applications, enabling precise colorimetric detection. It is primarily needed by manufacturers and research laboratories in the in-vitro diagnostics (IVD), biotechnology, and life sciences sectors.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): A key chromogenic substrate for horseradish peroxidase (HRP) in clinical diagnostics and research.
• Clinical Chemistry Analyzers: Used in automated systems for detecting biomarkers related to cardiac, liver, and metabolic functions.
• Point-of-Care Testing (POCT): Integral to rapid test strips and devices for on-site medical diagnostics.
• Immunohistochemistry (IHC): Employed as a staining reagent for visualizing tissue antigens in pathology.
• Molecular Biology Research: Serves as a detection reagent in blotting techniques (e.g., Western, Southern) and other enzyme-based assays.
• Food Safety & Environmental Testing: Utilized in kits for detecting pathogens, toxins, or contaminants via enzymatic methods.

Basic Information

Product Name	2,2'-Azino-Di-(3-Ethylbenzothiazoline)-6-Sulfonic Acid
CAS No.	28752-68-3
Molecular Formula	C18H24N6O6S4
Molecular Weight	548.68 g/mol
Synonyms	ABTS; 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid); Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate); ABTS Diammonium Salt; 2,2'-Azinobis(3-ethyl-2,3-dihydrobenzothiazole-6-sulfonic acid); 3-Ethyl-2,3-dihydrobenzothiazole-6-sulfonic acid azine; ABTS, diammonium salt
EINECS	Contact for details

Quality Control

Our 2,2'-Azino-Di-(3-Ethylbenzothiazoline)-6-Sulfonic Acid is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometric analysis for absorbance characteristics, and identity confirmation (IR). Certificates of Analysis (COA) with detailed specifications are provided to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	Green crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Absorbance (λ max)	340 - 345 nm (in buffer)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.