Description

4-Methylumbelliferyl β-D-Mannopyranoside is a specialized fluorogenic glycoside substrate designed for the sensitive detection and quantification of enzymatic activity. This compound is of critical importance in biochemical research and diagnostic assay development, where precise measurement of mannosidase and related glycosidase enzymes is required. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for applications in enzyme kinetics, high-throughput screening, and biomarker studies.

Application

• Enzyme Assay Substrate: A key fluorogenic substrate for the detection and kinetic analysis of α-mannosidase, β-mannosidase, and other glycosidases.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for the detection of lysosomal storage disorders, such as mannosidosis.
• Biochemical Research: Essential for studying glycosidase function, inhibition, and characterization in academic and industrial research laboratories.
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify potential therapeutic inhibitors of target glycosidase enzymes.
• Microbiology & Cell Biology: Useful for probing glycoprotein processing and carbohydrate metabolism in cellular and microbial systems.
• Quality Control in Bioprocessing: Applied in monitoring enzyme activity during the production and purification of recombinant proteins and biologics.

Basic Information

Product Name	4-Methylumbelliferyl β-D-Mannopyranoside
CAS No.	28541-83-5
Molecular Formula	C16H18O9
Molecular Weight	354.31 g/mol
Synonyms	4-MU-β-D-Mannopyranoside; 4-Methylumbelliferyl-β-D-mannopyranoside; β-4-Methylumbelliferyl mannopyranoside; 4-Methylumbelliferyl β-D-mannoside; 4-MU-β-Man; 4-Methyl-7-hydroxycoumarin β-D-mannopyranoside; 7-Hydroxy-4-methylcoumarin β-D-mannopyranoside
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl β-D-Mannopyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic identification (IR, NMR). Comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Functional in standard mannosidase assays
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.