Description

2'-Deoxy-5-Hydroxyuridine Triphosphate is a modified nucleotide triphosphate essential for advanced biochemical research and diagnostic development. This compound serves as a critical substrate in enzymatic studies, particularly for investigating DNA repair mechanisms and polymerase activity. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies focused on molecular biology, enzymology, and the development of novel diagnostic assays.

Application

• Enzymology Research: As a specialized substrate for studying the kinetics and fidelity of DNA polymerases and other nucleic acid-processing enzymes.
• DNA Repair Studies: Investigation of base excision repair (BER) pathways and the role of modified nucleotides in genomic stability.
• Biochemical Assay Development: A key component in the creation of sensitive in vitro diagnostic tests and high-throughput screening platforms.
• Antiviral & Anticancer Research: Exploration of its potential incorporation into nucleic acids for probing metabolic pathways or as a tool in targeted therapy research.
• Metabolic Pathway Analysis: Tracing nucleotide metabolism and incorporation in cellular systems.
• Synthesis of Modified Oligonucleotides: Used in enzymatic synthesis to produce probes and primers containing specific modifications for enhanced detection or stability.

Basic Information

Product Name	2'-Deoxy-5-Hydroxyuridine Triphosphate
CAS No.	27972-89-0
Molecular Formula	C10H15N2O15P3
Molecular Weight	504.15 g/mol
Synonyms	5-Hydroxy-2'-deoxyuridine 5'-triphosphate; 5-OH-dUTP; HO-dUTP; 5-Hydroxydeoxyuridine triphosphate; 2'-Deoxy-5-hydroxyuridine 5'-(tetrahydrogen triphosphate); d5-OHUTP; 5-Hydroxy-2'-deoxyuridine-5'-triphosphate; (2'R)-2'-Deoxy-5-hydroxyuridine 5'-(trihydrogen diphosphate) P'→5'-ester with phosphoric acid
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-5-Hydroxyuridine Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Solubility	Soluble in water, TE buffer
Water Content (KF)	≤ 5.0%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.