Description

5-Enolpyruvoylshikimate-3-Phosphate (CAS NO 27840-48-8) is a critical phosphorylated intermediate in the shikimate pathway, a fundamental metabolic route in plants, fungi, and bacteria. Its primary commercial and research significance lies in its role as the substrate for the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), the target of the widely used herbicide glyphosate. This compound is therefore essential for biochemical research, herbicide mechanism studies, and the development of novel antimicrobial agents targeting this pathway in pathogenic microbes.

Application

• Biochemical Research & Enzyme Studies: Used as a key substrate for investigating the kinetics, inhibition, and structure of EPSPS enzyme.
• Herbicide Mode-of-Action Research: Critical for studying the mechanism of glyphosate and developing next-generation herbicides with improved efficacy or novel modes of action.
• Antimicrobial Development: Serves as a target molecule for screening and designing new antibiotics and antifungal agents that disrupt the essential shikimate pathway in pathogens.
• Metabolic Pathway Analysis: Employed in metabolic engineering and systems biology to understand and manipulate aromatic amino acid biosynthesis in microorganisms and plants.
• Diagnostic Kit & Assay Development: Used in the formulation of high-purity kits for enzymatic activity assays and diagnostic testing in agricultural and life science laboratories.
• Reference Standard: Serves as a high-purity analytical standard for quality control in related pharmaceutical and agrochemical manufacturing processes.

Basic Information

Product Name	5-Enolpyruvoylshikimate-3-Phosphate
CAS No.	27840-48-8
Molecular Formula	C14H17NO11P2
Molecular Weight	437.23 g/mol
Synonyms	5-Enolpyruvylshikimate-3-phosphate; EPSP; 5-O-(1-Carboxyvinyl)-3-phosphoshikimic Acid; (5-Enolpyruvoyl-3-shikimic acid)-3-phosphate; Shikimate-3-phosphate 5-enolpyruvate; 3-Phospho-5-enolpyruvoylshikimate; 3-Phosphoshikimate 1-carboxyvinyltransferase substrate
EINECS	Contact for details

Quality Control

Our 5-Enolpyruvoylshikimate-3-Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) detailing batch-specific results are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Purity (HPLC, Area%)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.