Description

2'-Deoxyuridine, [5-3H] is a tritium-labeled nucleoside analog, a vital tool compound for biochemical and pharmacological research. Its core value lies in enabling precise tracking and quantification of DNA synthesis and repair mechanisms through radiometric detection. This labeled compound is essential for researchers in oncology, virology, and molecular biology who require high-specific-activity tracers for sensitive *in vitro* and *in vivo* studies.

Application

• DNA Synthesis & Cell Proliferation Assays: Used as a radiolabeled tracer to measure DNA replication rates and cell growth in cancer research and drug discovery.
• Thymidylate Synthase (TS) Inhibition Studies: A key substrate for evaluating the efficacy of chemotherapeutic agents like 5-fluorouracil that target this enzyme.
• Nucleoside Metabolism Research: Tracks the uptake, phosphorylation, and incorporation of deoxyuridine in metabolic pathway analysis.
• Antiviral Drug Mechanism Studies: Investigates the effect of antiviral compounds on viral DNA synthesis in herpesvirus and other DNA virus models.
• Autoradiography & Imaging: Serves as a probe for high-resolution localization of DNA synthesis sites in tissues and cell cultures.
• Biochemical Kit Component: Incorporated into commercial kits for the detection and study of enzymes involved in nucleotide metabolism.

Basic Information

Product Name	2'-Deoxyuridine, [5-3H]
CAS No.	27766-98-9
Molecular Formula	C9H113HN2O5
Molecular Weight	~229.2 g/mol (for unlabeled form)
Synonyms	5-Tritio-2'-deoxyuridine; [5-3H]dU; Thymidine precursor, tritiated; 1-(2-Deoxy-β-D-ribofuranosyl)-5-[3H]uracil; 2'-Deoxy-5-[3H]uridine; dU[5-3H]; Radiolabeled deoxyuridine; Tritiated dU
EINECS	Contact for details

Quality Control

Every batch of our 2'-Deoxyuridine, [5-3H] is manufactured and controlled under a strict quality management system. Radiochemical purity and specific activity are determined by validated methods including HPLC with radiometric detection. We provide comprehensive Certificates of Analysis (COA) detailing purity, concentration, specific activity, and solvent information to ensure traceability and reliability for your critical research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the solution at -20°C or below. Avoid repeated freeze-thaw cycles. All handling must be performed in accordance with local radiation safety regulations for tritium-labeled compounds.

Specification

Item	Specification
Appearance (Solution)	Clear, colorless to slightly yellow
Identification (HPLC)	Conforms to reference standard
Chemical Purity (HPLC)	≥ 98%
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	10-40 Ci/mmol (Contact for specific value)
Concentration	1 mCi/mL in ethanol:water (7:3) (or as specified)
Solvent System	Ethanol:Water (7:3 v/v)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.