Description

Hydroxycotinine is a major metabolite of nicotine, offering a critical analytical standard for research and diagnostic applications. Its high purity and stability make it an essential reference compound for accurate quantification in biological matrices. This product is primarily utilized by analytical laboratories, clinical research organizations, and pharmaceutical developers focused on nicotine metabolism studies, biomarker verification, and toxicology assessments.

Application

• Biomarker Analysis: Serves as a primary reference standard for quantifying nicotine metabolite levels in urine, blood, and saliva in clinical and epidemiological studies.
• Toxicology & Forensic Testing: Used as a calibrant in LC-MS/MS and GC-MS methods to determine tobacco exposure and for abstinence verification programs.
• Pharmacokinetic Research: Essential for studying the metabolic pathways, clearance rates, and bioavailability of nicotine in preclinical and clinical research.
• Diagnostic Kit Development: A key component in the manufacturing and calibration of immunoassay kits designed for nicotine metabolite detection.
• Reference Material for Quality Control: Employed by testing laboratories to validate analytical methods and ensure the accuracy and precision of their testing protocols.
• Academic Research: Supports fundamental research into the biological effects of nicotine and its metabolites on various physiological systems.

Basic Information

Item	Details
Product Name	Hydroxycotinine
CAS No.	27323-64-4
Molecular Formula	C10H12N2O2
Molecular Weight	192.22 g/mol
Synonyms	trans-3'-Hydroxycotinine; 5-(3-Hydroxy-1-methyl-2-pyrrolidinyl)-2-pyridinol; 3-Hydroxycotinine; Cotinine Metabolite; Hydroxycotinine (major nicotine metabolite); 1-Methyl-5-(3-pyridyl)-2-pyrrolidinone-3-ol; Norcotinine carboxylic acid lactone derivative
EINECS	Contact for details

Quality Control

Our Hydroxycotinine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for analytical applications. Each lot undergoes comprehensive testing, including identity confirmation by spectroscopic methods and purity assessment by chromatographic techniques. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.