Description

4-Nitrophenyl3-o-(B-D-Glucopyranosyl)-B-D-Glucopyranoside is a specialized synthetic glycoside derivative, primarily utilized as a chromogenic substrate for enzymatic assays. This compound is critical for research and quality control in biochemistry and molecular biology, where precise measurement of enzymatic activity is required. It is an essential reagent for laboratories and manufacturers in the pharmaceutical, diagnostic, and life science research sectors.

Application

• Enzymatic Substrate: Primary use as a chromogenic substrate for β-glucosidase and related glycosidase enzymes in biochemical assays.
• Diagnostic Kits: A key component in diagnostic test kits for measuring enzyme levels in clinical and research samples.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of glycoside hydrolase activity.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms during drug discovery for identifying enzyme modulators.
• Quality Control: Serves as a reference standard for the quality assurance of enzyme preparations and diagnostic reagents.
• Microbiology: Applied in assays to study microbial enzyme systems and carbohydrate metabolism.

Basic Information

Product Name	4-Nitrophenyl3-o-(B-D-Glucopyranosyl)-B-D-Glucopyranoside
CAS No.	26255-70-9
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	4-Nitrophenyl β-D-Gentiobioside; p-Nitrophenyl β-D-Gentiobioside; p-Nitrophenyl β-Gentiobioside; 4-NPG; PNPG2; 4-Nitrophenyl β-D-Glucosyl-(1→6)-β-D-glucopyranoside; 4-Nitrophenyl 6-O-β-D-glucopyranosyl-β-D-glucopyranoside; 4-Nitrophenyl β-Gentiobioside
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl3-o-(B-D-Glucopyranosyl)-B-D-Glucopyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive analytical applications. Each lot undergoes comprehensive analysis, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.