Description

2,4-Dinitrophenyl β-D-Galactoside Tetraacetate is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of β-galactosidase activity, serving as a key reagent in molecular biology and diagnostic applications. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers for applications in gene expression studies, reporter assays, and enzyme kinetics.

Application

• β-Galactosidase Assay Substrate: Acts as a chromogenic substrate for the colorimetric detection and measurement of β-galactosidase enzyme activity in reporter gene assays (e.g., lacZ gene expression).
• Molecular Biology Research: Essential tool in cloning, transfection efficiency studies, and promoter characterization in bacterial, yeast, and mammalian cell systems.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for microbiology and clinical chemistry to identify specific bacterial enzymes.
• Enzyme Kinetics & Inhibition Studies: Employed in biochemical research to study enzyme mechanisms, calculate kinetic parameters (Km, Vmax), and screen for potential inhibitors.
• High-Throughput Screening (HTS): Suitable for use in automated screening platforms within pharmaceutical R&D for drug discovery campaigns targeting glycosidase enzymes.
• Biochemical Reagent: Serves as a critical component in buffer formulations for standardizing enzymatic reactions in quality control laboratories.

Basic Information

Product Name	2,4-Dinitrophenyl β-D-Galactoside Tetraacetate
CAS No.	25775-99-9
Molecular Formula	C22H24N2O15
Molecular Weight	556.43 g/mol
Synonyms	2,4-Dinitrophenyl β-D-galactopyranoside tetraacetate; DNP-β-D-Galactopyranoside tetraacetate; O-(2,4-Dinitrophenyl)-β-D-galactopyranoside tetraacetate; β-D-Galactopyranoside, 2,4-dinitrophenyl, tetraacetate; 2,4-DNPGal tetraacetate; Galactose-β-1-O-(2,4-dinitrophenyl) tetraacetate; DNP-Gal-β-O-tetraacetate
EINECS	Contact for details

Quality Control

Our 2,4-Dinitrophenyl β-D-Galactoside Tetraacetate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification (NMR, IR). A detailed Certificate of Analysis (COA) providing identity, purity, and impurity profile is supplied with every shipment to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	158 - 162 °C
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.