Description

2-Nitrophenyl-β-D-Galactopyranoside-6-Phosphate Dicyclohexyl is a specialized chromogenic and fluorogenic substrate used in enzymatic assays. This compound is critical for the sensitive and specific detection of enzymatic activity, particularly for phosphatases and glycosidases. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic kit manufacturing industries for developing and validating biochemical assays.

Application

• Enzyme Activity Assays: As a key substrate for detecting and quantifying the activity of alkaline phosphatase (ALP) and related enzymes in research and clinical diagnostics.
• Diagnostic Kit Development: Used in the formulation of ELISA kits, Western blotting detection systems, and other in-vitro diagnostic (IVD) reagents.
• High-Throughput Screening (HTS): Employed in pharmaceutical discovery for screening compound libraries against specific enzymatic targets.
• Biochemical Research: A vital tool for studying enzyme kinetics, inhibition mechanisms, and signal transduction pathways in molecular biology.
• Microbiology: Can be used in assays to study bacterial gene expression (e.g., lacZ reporter systems) where specific glycosidase activity is a marker.
• Quality Control Testing: For the validation and lot-release testing of therapeutic enzymes and other biopharmaceutical products.

Basic Information

Product Name	2-Nitrophenyl-β-D-Galactopyranoside-6-Phosphate Dicyclohexyl
CAS No.	25405-62-3
Molecular Formula	C24H36NO12P
Molecular Weight	561.52 g/mol
Synonyms	PNPG-6-P Dicyclohexylammonium Salt; 2-Nitrophenyl β-D-galactopyranoside 6-(dihydrogen phosphate) dicyclohexylammonium salt; o-Nitrophenyl-β-D-galactopyranoside 6-phosphate DCHA salt; ONPG-6-P DCHA salt; β-Galactosidase substrate 6-phosphate; 6-Phospho-2-nitrophenyl-β-D-galactopyranoside dicyclohexylamine salt; Alkaline phosphatase chromogenic substrate.
EINECS	Contact for details

Quality Control

Our 2-Nitrophenyl-β-D-Galactopyranoside-6-Phosphate Dicyclohexyl is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your regulatory and research needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and performance. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.