Description

3-Methyl-3H-Diazirine-3-Propionyl Chloride is a highly reactive, heterocyclic acid chloride derivative that serves as a critical photoactivatable crosslinking agent. Its core value lies in its ability to form covalent bonds upon UV light irradiation, enabling the study of biomolecular interactions and the stabilization of protein complexes. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and advanced materials science who require precise, irreversible labeling and crosslinking capabilities.

Application

• Photoaffinity Labeling (PAL): A key reagent for identifying and characterizing low-affinity protein-ligand interactions and binding sites in drug discovery.
• Bioconjugation & Protein Crosslinking: Used to create stable covalent links between biomolecules for applications in antibody-drug conjugate (ADC) development and biosensor fabrication.
• Proteomics Research: Enables the capture and analysis of transient protein-protein interactions and complex structural mapping.
• Surface Immobilization: Facilitates the covalent attachment of biomolecules to chips, beads, and other solid supports for diagnostic assays and microarray technology.
• Polymer & Materials Science: Acts as a functional monomer or crosslinker for synthesizing photo-responsive polymers and smart materials.
• Chemical Biology Tool Development: Integral for creating probes to study enzyme mechanisms, metabolic pathways, and cellular processes.

Basic Information

Product Name	3-Methyl-3H-Diazirine-3-Propionyl Chloride
CAS No.	25055-90-7
Molecular Formula	C5H7ClN2O
Molecular Weight	146.57 g/mol
Synonyms	3-(3-Methyl-3H-diazirin-3-yl)propanoyl chloride; Diazirine-3-propionyl chloride, 3-methyl-; 3-Methyl-3H-diazirine-3-propanoic acid chloride; Photoactivatable crosslinker; PAL reagent; 3-Methyl-diazirine-propionyl chloride; 3-(3-Methyldiazirin-3-yl)propionyl chloride
EINECS	Contact for details

Quality Control

Our production of 3-Methyl-3H-Diazirine-3-Propionyl Chloride adheres to stringent in-process controls to ensure batch-to-batch consistency and high chemical purity. Every lot is characterized by multiple analytical techniques, including NMR, HPLC, and IR spectroscopy, to confirm identity and assess critical impurities. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substance data is provided with each shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at or below -20°C under an inert atmosphere (e.g., argon or nitrogen) due to its moisture-sensitive and thermally sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis. Keep away from strong bases and oxidizing agents.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid or low-melting solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 97%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.