Description

H-Glu-Pna is a high-purity biochemical reagent and a derivative of glutamic acid, widely used as a key substrate in enzymatic assays and proteomics research. Its primary value lies in its role as a chromogenic substrate for the detection and quantification of protease activity, particularly for enzymes like glutamyl endopeptidase. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic kit development sectors who require reliable and sensitive tools for enzyme kinetics and inhibitor screening studies.

Application

• Protease Activity Assays: Serves as a specific chromogenic substrate for glutamyl-specific proteases, enabling the measurement of enzyme kinetics and inhibition.
• Biochemical Research: Used in academic and industrial R&D for studying protease function, substrate specificity, and mechanism of action.
• Diagnostic Reagent Manufacturing: A critical component in the formulation of diagnostic kits for detecting specific protease activities related to various physiological or pathological conditions.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential drug candidates.
• Quality Control in Bioprocessing: Used to monitor and validate the activity of proteolytic enzymes used in biomanufacturing processes.

Basic Information

Product Name	H-Glu-Pna
CAS No.	24032-35-7
Molecular Formula	C11H13N3O4
Molecular Weight	251.24 g/mol
Synonyms	L-Glutamic acid 4-nitroanilide; N-(4-Nitrophenyl)-L-glutamine; L-Glutamic acid γ-(4-nitroanilide); Glutaryl-L-phenylalanine 4-nitroanilide; H-Glu-pNA; Glu-pNA; Substrate for Glu-specific protease; Protease substrate 24032-35-7
EINECS	Contact for details

Quality Control

Our H-Glu-Pna is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification, identity confirmation by IR and NMR, and specific optical rotation measurement. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after each use to prevent absorption of atmospheric moisture, which could affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.