Description

2'-Deoxyadenylyl-(3'-5')-Thymidine is a defined dinucleotide, a fundamental building block in nucleic acid research and synthesis. This compound is critical for studying DNA structure, function, and enzymatic processes, providing a precise model for oligonucleotide interactions. It is primarily utilized by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in molecular biology, diagnostics development, and antisense therapy research.

Application

• Oligonucleotide Synthesis: Serves as a key intermediate or reference standard in the chemical and enzymatic synthesis of DNA strands.
• Biochemical Research: Used as a substrate or model compound for studying DNA polymerases, nucleases, and other DNA-modifying enzymes.
• Structural Studies: Employed in NMR and X-ray crystallography to investigate DNA conformation, base pairing, and stacking interactions.
• Diagnostic Probe Development: Acts as a core component in the design and calibration of hybridization probes for genetic testing.
• Antisense & Aptamer Research: Provides a defined sequence for evaluating binding affinity and specificity in therapeutic oligonucleotide discovery.
• Teaching & Reference Standard: Used in academic settings for advanced biochemistry education and as a high-purity calibrant in analytical laboratories.

Basic Information

Item	Details
Product Name	2'-Deoxyadenylyl-(3'-5')-Thymidine
CAS No.	23339-47-1
Molecular Formula	C20H25N7O11
Molecular Weight	539.46 g/mol
Synonyms	d(ApT); Deoxyadenylyl-(3'→5')-thymidine; Adenine deoxyribosylthymidine dinucleotide; d-adenylyl-(3'-5')-thymidine; ApT; 2'-Deoxyadenosine 5'-(trihydrogen diphosphate), 5'-5' ester with thymidine; Deoxyadenosine-thymidine dinucleotide; pd(A-T)
EINECS	Contact for details

Quality Control

Every batch of 2'-Deoxyadenylyl-(3'-5')-Thymidine is manufactured and tested under stringent quality management protocols to ensure identity, purity, and consistency suitable for sensitive research applications. Our standard analytical suite includes HPLC for purity assessment, NMR and MS for structural confirmation, and tests for moisture content. Certificates of Analysis (COA) documenting all release specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.