Description

4-Nitrophenyl 2-Acetamido-3-o-acetyl-2-deoxy-β-D-glucopyranoside is a specialized glycoside derivative, primarily utilized as a chromogenic substrate for enzymatic assays. This compound is critical for biochemical research and diagnostic applications, offering high sensitivity and specificity for detecting enzyme activity. It is an essential reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors.

Application

• Enzyme Substrate: Acts as a chromogenic substrate for β-N-acetylglucosaminidase and related glycosidase enzymes in kinetic studies and activity assays.
• Biochemical Research: Used in the study of carbohydrate metabolism, enzyme mechanisms, and inhibitor screening in academic and industrial laboratories.
• Diagnostic Kits: Incorporated into in-vitro diagnostic (IVD) kits for the detection and quantification of specific enzyme markers in clinical samples.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms during drug discovery to identify potential therapeutic agents targeting glycosidases.
• Quality Control: Serves as a reference standard in the quality control of enzyme preparations and diagnostic reagents.

Basic Information

Product Name	4-Nitrophenyl 2-Acetamido-3-o-acetyl-2-deoxy-β-D-glucopyranoside
CAS No.	23262-57-9
Molecular Formula	C₁₇H₂₂N₂O₉
Molecular Weight	398.37 g/mol
Synonyms	p-Nitrophenyl N-acetyl-β-D-glucosaminide 3-acetate; p-Nitrophenyl 2-acetamido-2-deoxy-3-O-acetyl-β-D-glucopyranoside; 4-Nitrophenyl 3-O-acetyl-2-acetamido-2-deoxy-β-D-glucopyranoside; PNP-3-O-Ac-GlcNAc; 3-O-Acetyl-PNP-β-GlcNAc; β-GlcNAcase substrate 3-acetate; NAG-3-OAc-pNP; 2-Acetamido-2-deoxy-3-O-acetyl-β-D-glucopyranosyl 4-nitrophenyl ester
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 2-Acetamido-3-o-acetyl-2-deoxy-β-D-glucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.