Description

4-Methylumbelliferyl Phosphate Disodium Salt is a high-purity, synthetic fluorogenic substrate widely used in enzymatic assays. Its core value lies in its ability to generate a highly fluorescent product upon dephosphorylation by enzymes like alkaline phosphatase, enabling sensitive detection and quantification. This compound is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors, where precise enzymatic activity measurement is critical.

Application

• Primary Substrate for Phosphatase Assays: A standard fluorogenic substrate for detecting and quantifying the activity of alkaline phosphatase (ALP) and other phosphatases in biochemical research and diagnostic kits.
• Diagnostic Reagent Manufacturing: Used in the production of clinical diagnostic reagents for measuring enzyme levels in serum, which are important biomarkers for liver and bone diseases.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research for HTS assays to identify enzyme inhibitors or activators in drug discovery programs.
• Cell Biology & Histochemistry: Utilized as a substrate in fluorescence microscopy and flow cytometry for detecting phosphatase activity within cells and tissues.
• Immunoassay Development: Serves as a key component in enzyme-linked immunosorbent assays (ELISAs) and other immunoassays that use alkaline phosphatase as a reporter enzyme.
• Water & Environmental Testing: Applied in microbial detection assays where phosphatase activity is used as an indicator of bacterial contamination.
• Quality Control in Biotechnology: Used to standardize and validate the activity of enzyme preparations and diagnostic kits, ensuring batch-to-batch consistency.

Basic Information

Product Name	4-Methylumbelliferyl Phosphate Disodium Salt
CAS No.	22919-26-2
Molecular Formula	C10H7Na2O7P
Molecular Weight	316.11 g/mol
Synonyms	4-MUP; 4-Methylumbelliferyl phosphate, disodium salt; 4-Methylumbelliferyl phosphate sodium salt; 4-MU-P; 7-Hydroxy-4-methylcoumarin phosphate disodium salt; 4-Methylumbelliferyl dihydrogen phosphate disodium salt; Phosphoric acid mono-(4-methyl-2-oxo-2H-chromen-7-yl) ester disodium salt
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Phosphate Disodium Salt is manufactured under strict quality management systems to ensure high purity and consistent performance. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectroscopic identification, and functional enzymatic activity validation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorescence Test	Conforms (Suitable for enzymatic assays)
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.