Description

4-Nitrophenyl-β-L-Fucopyranoside is a specialized chromogenic substrate designed for the detection and quantification of specific glycosidase enzyme activity. This compound matters for its high sensitivity and specificity in biochemical assays, enabling precise research and diagnostic applications. It is primarily needed by biochemical researchers, diagnostic kit manufacturers, and pharmaceutical companies involved in enzyme characterization and inhibitor screening.

Application

• Enzymatic Substrate for α-L-Fucosidase: A critical chromogenic substrate used in clinical and research laboratories to assay the activity of α-L-fucosidase enzymes.
• Diagnostic Reagent Manufacturing: A key raw material in the production of diagnostic kits for metabolic disorders, such as fucosidosis.
• Biochemical Research & Enzyme Kinetics: Used to study enzyme mechanisms, inhibition, and kinetics in vitro for research purposes.
• Microbiology & Cell Biology: Employed in assays to detect bacterial or cellular fucosidase activity as a phenotypic marker.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential glycosidase inhibitors for drug discovery.
• Quality Control in Bioprocessing: Applied to monitor enzyme activity and consistency in biomanufacturing processes.

Basic Information

Product Name	4-Nitrophenyl-β-L-Fucopyranoside
CAS No.	22153-71-5
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	p-Nitrophenyl β-L-fucopyranoside; 4-Nitrophenyl β-L-fucopyranoside; PNPGal (context-specific); β-L-Fucopyranoside, 4-nitrophenyl; p-Nitrophenyl-β-L-fucoside; 4-Nitrophenyl fucoside; L-Fucose 4-nitrophenyl glycoside; Fucosidase substrate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-β-L-Fucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which may affect performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	≥ 97.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.